The acetylene reduction technique as an assay for nitrogenase activity in the methane oxidizing bacterium Methylococcus capsulatus strain bath
| Autor: | Roger Whittenbury, Howard Dalton |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
biology
Methane monooxygenase Inorganic chemistry Substrate (chemistry) Nitrogenase General Medicine biology.organism_classification Biochemistry Microbiology Methane chemistry.chemical_compound Methylococcus chemistry Acetylene Genetics biology.protein Vanadium nitrogenase Molecular Biology Methylococcus capsulatus |
| Zdroj: | Archives of Microbiology. 109:147-151 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/bf00425127 |
| Popis: | The use of acetylene as a convenient assay substrate for nitrogenase in methane oxidising bacteria is complicated by the observation that it is a potent inhibitor of the methane monooxygenase enzyme in both whole cells and cell-free extracts. If the cells were provided with alternative oxidisable carbon substrates other than methane then nitrogen fixing cells would reduce acetylene to ethylene. Hydrogen gas also served as an oxidisable substrate in the assay. Nitrous oxide, which is reduced by nitrogenase to N2 and H2O, was not an inhibitor of methane monooxygenase function and could be used as a convenient assay substrate for nitrogenase. Reduction of both substrates by whole cells showed similar response to oxygen in the assay system and in this respect Methylococcus resembles other free living nitrogen fixing aerobes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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