Ribosome Profiling in Trypanosomatids

Autor:	Raul O Cosentino, Amelie J. Kraus
Rok vydání:	2019
Předmět:	0303 health sciences Chemistry 030303 biophysics RNA Computational biology Ribosome Deep sequencing 03 medical and health sciences chemistry.chemical_compound Gene expression Protein biosynthesis Nuclease digestion Ribosome profiling DNA 030304 developmental biology
Zdroj:	Methods in Molecular Biology ISBN: 9781493992096
DOI:	10.1007/978-1-4939-9210-2_5
Popis:	Ribosomes are the machinery responsible for reading mRNAs and translating them into proteins. The ribosome profiling approach is based on high-throughput sequencing of ribosome-protected mRNAs. RNAs not harboring ribosomes are removed by nuclease digestion leaving the so-called ribosome "footprints." The purified "footprint" RNA molecules are processed into DNA libraries and their individual abundance is determined by deep sequencing. Ribosome profiling reveals the portion of transcripts which are actually protein-coding and can be used for differential gene expression analysis addressing rates of protein synthesis, and translational control and efficiency.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6406fd3837770a70816c312da704da71 https://doi.org/10.1007/978-1-4939-9210-2_5 Zobrazit plný text záznamu