Development and characterization of SSR markers in Ribes species

Autor:	Rex M. Brennan, Joanne Russell, M. Woodhead, Linzi Jorgensen
Rok vydání:	2002
Předmět:	Genetics Germplasm Genetic diversity Ecology biology Ribes biology.organism_classification Biochemistry General Biochemistry Genetics and Molecular Biology Genus Genetic marker Microsatellite Allele Repeated sequence
Zdroj:	Molecular Ecology Notes. 2:327-330
ISSN:	1471-8286 1471-8278
DOI:	10.1046/j.1471-8286.2002.00233.x
Popis:	Eleven microsatellite loci were identified and characterized in blackcurrant (Ribes nigrum L.) and related species. An enriched library was constructed and screened with simple sequence repeat (SSR) oligonucleotides. Positive clones were sequenced and primers were designed flanking the repeat motifs. These 11 microsatellites produce amplification products polymorphic across a range of Ribes germplasm, predominantly from the Eucoreosma section of the genus. The number of alleles varied from 2 to 18 with levels of diversity ranging from 0.18 to 0.91.
Databáze:	OpenAIRE
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