Evaluation of different temperature-sensitive mutant phenotypes ofSalmonella enteritidis as vaccine potentials

Autor:	M. Magdalena Gherardi, Daniel O. Sordelli, M. Cristina Cerquetti
Rok vydání:	1990
Předmět:	biology Salmonella enteritidis Mutant Reversion Mutagenesis (molecular biology technique) General Medicine biology.organism_classification Temperature-sensitive mutant Applied Microbiology and Biotechnology Microbiology Enterobacteriaceae Virology Antigen Humoral immunity
Zdroj:	Current Microbiology. 21:225-228
ISSN:	1432-0991 0343-8651
DOI:	10.1007/bf02092160
Popis:	Temperature-sensitive (ts) mutants ofSalmonella enteritidis were isolated after mutagenesis with UV light and enrichment with antibiotic. Mutants were characterized according to their growth profile at the permissive (28°C) and the nonpermissive (37°C) temperatures, persistence of surface antigens, reversion frequencies, and potentials for inducing humoral immunity and protection against challenge with the parental wild-type (wt) in mice. We obtained 32 strains ofS. enteritidis able to grow well at 28°C, but capable of only limited or no replication at 37°C. The ts mutants were positive for factor 9 in an agglutination assay and were susceptible to infection with phage P22. Three mutants of different phenotypes were selected for protection studies. A single intraperitoneal (i.p.) immunization with any of the mutants studied induced significant protection from i.p. challenge with 100 LD50 of the wt strain.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::63f5bfa4acd2afb0db5d058ebe67ed33 https://doi.org/10.1007/bf02092160 Zobrazit plný text záznamu Full text from SpringerLink