Optimizing combination of vascular endothelial growth factor and mesenchymal stem cells on ectopic bone formation in SCID mice
| Autor: | Nicholas Ditzel, Chris Halling Dreyer, Ming Ding, Niklas Rye Jørgensen, Søren Overgaard, Kristian Kjærgaard |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology Angiogenesis Mesenchymal stem cell Metals and Alloys Biomedical Engineering Vimentin Staining Biomaterials Andrology Vascular endothelial growth factor 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Tissue engineering chemistry 030220 oncology & carcinogenesis Immunology Ceramics and Composites biology.protein Immunohistochemistry Bone regeneration |
| Zdroj: | Journal of Biomedical Materials Research Part A. 105:3326-3332 |
| ISSN: | 1549-3296 |
| Popis: | Introduction: Insufficient blood supply may limit bone regeneration in bone defects. Vascular endothelial growth factor (VEGF) promotes angiogenesis by increasing endothelial migration. This outcome, however, could depend on time of application. Sheep mesenchymal stem cells (MSCs) in severe combined immunodeficient (SCID) mice were used in this study to evaluate optimal time points for VEGF stimulation to increase bone formation. Methods: Twenty-eight SCID (NOD.CB17-Prkdcscid/J) mice had hydroxyapatite (HA) granules seeded with 5x105 MSCs inserted subcutaneously. Pellets released VEGF on Days 1-7, Days 1-14, Days 1-21, Days 1-42, Days 7-14, and Days 21-42. After 8 weeks, the implant-bone-blocks were harvested, paraffin embedded, sectioned, and stained with both Haematoxylin Eosin (HE) and immunohistochemistry for human vimentin staining (hVim). Blood samples were collected for determination of bone-related biomarkers in serum. Results: The groups with 5x105 mesenchymal stem cells and VEGF stimulation on Days 1-14 and Days 1-21 showed more bone formation when compared to the control group of 5x105 mesenchymal stem cells alone (p |
| Databáze: | OpenAIRE |
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