Examination the Hydrolysis Feasibility of OPEFB Biomass Using Aspergillus niger as Cellulase Enzyme-producing Fungus
| Autor: | Muhammad Nurdin, L O A Salim, Maulidiyah Maulidiyah, Dwiprayogo Wibowo, Haznan Abimanyu, Zaenal Syam Arifin, Muhammad Naufalsar |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences biology 030309 nutrition & dietetics General Chemical Engineering Aspergillus niger Biomass 04 agricultural and veterinary sciences General Medicine General Chemistry Cellulase biology.organism_classification Pulp and paper industry 040401 food science Reducing sugar 03 medical and health sciences Hydrolysis 0404 agricultural biotechnology chemistry Palm oil biology.protein |
| Zdroj: | Journal of Oleo Science. 70:637-645 |
| ISSN: | 1347-3352 1345-8957 |
| Popis: | The objective of this study was to obtain optimization results from the biological hydrolysis of Oil Palm Empty Fruit Bunches (OPEFB) using Aspergillus niger (A. niger) BIOTROP 2173 isolated from grain. Optimized hydrolysis parameters include temperature, pH and time. The hydrolysis process was carried out by growing A. niger on OPEFB powder (± 30 mesh) through two schemes, namely hydrolysis on OPEFB pretreatment with 10% NaOH and hydrolysis on OPEFB non-pretreatment. The optimization results show that the best hydrolysis process of A. niger BIOTROP 2173 occurs in OPEFB pretreatment. The optimum conditions for temperature, pH and time obtained are 40°C, 6 and 24 hours, respectively. Although the amount of reducing sugar produced was lower than the OPEFB non-pretreatment, the performance of the cellulase enzyme during the hydrolysis process of OPEFB pretreatment was very good, with a fast hydrolysis rate. These results indicate that the performance of A. niger BIOTROP 2173 in the hydrolysis process is influenced by the pretreatment stage. The optimum conditions obtained then became a reference in the production of reducing sugar based on A. niger BIOTROP 2173. The amount of reducing sugar produced from OPEFB pretreatment was 0.94 mg.mL-1, while for OPEFB non-pretreatment was 15.83 mg.mL-1. |
| Databáze: | OpenAIRE |
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