The solid-state proton NMR study of bone using a dipolar filter: apatite hydroxyl contentversusanimal age
| Autor: | Agnieszka Kaflak, Waclaw Kolodziejski, Stanislaw Moskalewski |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Bone mineral
Proton General Chemical Engineering Inorganic chemistry 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Apatite 0104 chemical sciences Hydroxylation chemistry.chemical_compound chemistry visual_art Magic angle spinning Proton NMR visual_art.visual_art_medium 0210 nano-technology Quantitative analysis (chemistry) Stoichiometry |
| Zdroj: | RSC Advances. 9:16909-16918 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/c9ra01902b |
| Popis: | The hydroxyl content of bone apatite mineral has been measured using proton solid-state NMR performed with a multiple-pulse dipolar filter under slow magic angle spinning (MAS). This new method succeeded in resolving and relatively enhancing the main hydroxyl peak at ca. 0 ppm from whole bone, making it amenable to rigorous quantitative analysis. The proposed methodology, involving line fitting, the measurement of the apatite concentration in the studied material and adequate calibration, was proved to be convenient and suitable for monitoring bone mineral hydroxylation in different species and over the lifetime of the animal. It was found that the hydroxyl content in the cranial bone mineral of pig and rats remained in the 5–10% range, with reference to stoichiometric hydroxyapatite. In rats, the hydroxyl content showed a non-monotonic increase with age, which was governed by biological processes rather than by chemical, thermodynamically driven apatite maturation. |
| Databáze: | OpenAIRE |
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