Predominant Shift of Different P44-Expressing Anaplasma phagocytophilum in Infected HL-60, THP-1, NB4, and RF/6A Cell Lines
| Autor: | Yuko Shimamura, Norio Ohashi, Naoya Takamoto, Masahiko Shimada, Haruka Sasahara, Hongru Su, Shuji Ando |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) endocrine system Human granulocytic anaplasmosis 030106 microbiology RNA-Seq environment and public health 03 medical and health sciences 0302 clinical medicine Antigen parasitic diseases medicine Intracellular bacterium THP1 cell line 030212 general & internal medicine biology General Medicine biology.organism_classification medicine.disease Anaplasma phagocytophilum Molecular biology enzymes and coenzymes (carbohydrates) Infectious Diseases Cell culture biological phenomena cell phenomena and immunity Bacterial outer membrane |
| Zdroj: | Japanese Journal of Infectious Diseases. 72:73-80 |
| ISSN: | 1884-2836 1344-6304 |
| DOI: | 10.7883/yoken.jjid.2018.230 |
| Popis: | Anaplasma phagocytophilum, an agent of human granulocytic anaplasmosis, is an obligatory intracellular bacterium that dominantly produces P44 outer membrane proteins encoded by the p44/msp2 multigene family, which are major antigens for serodiagnosis. However, A. phagocytophilum antigens from cultures with different cell lines seem to have varying reactivities with sera. In this study, we performed RNA-seq to investigate the P44 expression of A. phagocytophilum propagated in 4 cell lines. In infected HL-60 cells, the P44-2b transcript was predominant in the first RNA-seq analysis (HL-60.1). However, the P44-23 transcript was predominant in the second RNA-seq analysis at 1 month after additional passages (HL-60.2). We further analyzed the P44 expression of A. phagocytophilum cultured in THP-1, NB4, and RF/6A cells through consecutive passages in the same cell lines for 1 year after transferring A. phagocytophilum from infected HL-60 cells to the respective cell lines. In the long-term cultures, P44-18, P44-78, and P44-51 were predominantly transcribed in infected THP-1, NB4, and RF/6A cells, respectively. Therefore, the predominant shifts of different P44-expressing transcripts of A. phagocytophilum might occur during cell culture even in the same cell line at different time points of sample harvest (HL-60.1 and HL-60.2), which may be attributed to host cell adaptation/selection/interaction. |
| Databáze: | OpenAIRE |
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