A solution of identifying biophysical properties and 3D cellular structure of visible-light-driven photocatalytic inactivated Staphylococcus aureus
| Autor: | Li-Ting Yen, Mon-Shu Ho, Jian-Yu Chen, Gulomjon Gaybullaev, Jing-Hua Tzeng, Yao-Tung Lin, Chun-Chieh Wang, Chakkrit Poonpakdee, Chih-Huang Weng |
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| Rok vydání: | 2021 |
| Předmět: |
General Chemical Engineering
Cell technology industry and agriculture 02 engineering and technology General Chemistry Bacterial growth 010402 general chemistry 021001 nanoscience & nanotechnology medicine.disease 01 natural sciences Industrial and Manufacturing Engineering 0104 chemical sciences Lipid peroxidation Cell membrane chemistry.chemical_compound medicine.anatomical_structure chemistry Microscopy medicine Photocatalysis Biophysics Environmental Chemistry 0210 nano-technology Cell adhesion Cell damage |
| Zdroj: | Chemical Engineering Journal. 421:127880 |
| ISSN: | 1385-8947 |
| DOI: | 10.1016/j.cej.2020.127880 |
| Popis: | The mechanism of bacterial damage under visible-light-driven photo-inactivation system was attempted. Atomic force microscopy (AFM) and transmission X-ray microscopy (TXM) were firstly used to identify changes in biophysical properties, such as cellular height, roughness, adhesion, and modulus, and 3D cellular structure of microbial cells under visible-light-responsive N-TiO2 inactivation. Results revealed that the cell adhesion of N-TiO2 occurred immediately upon light irradiation then N-TiO2 particles penetrated the cell membrane, and deformed the cellular structure at the beginning of log phase under photocatalytic inactivation. The destruction of the outer cellular caused leakage of cellular K+ and lipid peroxidation, which ultimately brought about severe decrease in cell density. AFM and TXM provided direct observations on interactions between single cell and nano-materials at the nanoscale, which was useful for the early diagnosis of cell damage. |
| Databáze: | OpenAIRE |
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