Role of Vector in The Dynamics of Antisense Activity

Autor:	Srikanth Sundaram, Li Kim Lee, Charles M. Roth
Rok vydání:	2006
Předmět:	chemistry.chemical_classification Polyethylenimine Molecular mass Chemistry Oligonucleotide Dynamics (mechanics) Cationic polymerization hemic and immune systems Polymer respiratory system chemistry.chemical_compound Biochemistry Biophysics DNA
Zdroj:	Proceedings of the IEEE 32nd Annual Northeast Bioengineering Conference.
DOI:	10.1109/nebc.2006.1629812
Popis:	The objective of this work is to investigate the role of the vector in the dynamics of antisense effects. Polymer/DNA complexs between cationic, branched polymer, polyethylenimine (PEI) at a range of molecular weights and oligonucleotides (ODNs), with varying backbone chemistries, were studied using a variety of physico-chemical and cellular assays. The extent and time scale of antisense effects were found to depend strongly on both the polymer molecular weight as well as the ODN chemistry. The optimum molecular weight of PEI for maximum ODN delivery depends strongly on the ODN chemistry.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::63ae4ee5629f65e301f1d0d68cffcf12 https://doi.org/10.1109/nebc.2006.1629812 Zobrazit plný text záznamu