Studies on Marsupial Proteins III. N-Bromosuccinimide Cleavage of the (?-Chain of Kangaroo Haemoglobin and the Amino Acid Sequence of the N-Terminal Fragment
| Autor: | Thompson Eo, Beard Jm |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Size-exclusion chromatography
Tryptophan General Medicine Biology Cleavage (embryo) chemistry.chemical_compound Endocrinology Reproductive Medicine chemistry Biochemistry Genetics Urea General Materials Science Animal Science and Zoology Titration N-Bromosuccinimide Molecular Biology Peptide sequence Alpha chain Developmental Biology Biotechnology |
| Zdroj: | Australian Journal of Biological Sciences. 23:185 |
| ISSN: | 0004-9417 |
| DOI: | 10.1071/bi9700185 |
| Popis: | The tryptophan contents of the (X- and ,a-chains of haemoglobin from the grey kangaroo Macropus giganteUB were determined by N-bromosuccinimide titration to be 1 and 2 residues respectively. Following cleavage of the tryptophyl bond in the (X-chain with N-bromosuccinimide in 8M urea solutions at pH 3�5 the N-termina.l fragment was purified by gel filtration and paper ionophoresis. |
| Databáze: | OpenAIRE |
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