Groundnut (Apios americana Medik) Extract Enhances the Osteoblast Differentiation of MC3T3-E1 Cells
| Autor: | Sakiko Sugino, Atsumi Tada, Kazuhiro Nara, Masanori Horie |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
musculoskeletal diseases
0106 biological sciences Pharmacology 0303 health sciences biology food and beverages Osteoblast Plant Science General Medicine Apios biology.organism_classification Mc3t3 e1 01 natural sciences Cell biology 03 medical and health sciences medicine.anatomical_structure stomatognathic system Complementary and alternative medicine 010608 biotechnology Drug Discovery medicine 030304 developmental biology |
| Zdroj: | Natural Product Communications. 16:1934578X2110105 |
| ISSN: | 1555-9475 1934-578X |
| DOI: | 10.1177/1934578x211010567 |
| Popis: | The effects of groundnut ( Apios americana Medik) extract on osteoblast differentiation were examined using MC3T3-E1 cells. MC3T3-E1 cells were treated with the crude extract along with other differentiating reagents. The alkaline phosphatase (ALP) activity of cells cultured in a differentiation medium supplemented with 0.01% crude groundnut extract was 1.5‐1.6 times higher than that of cells cultured in a differentiation medium without the extract. Crude groundnut extract was further separated into aqueous and methanol fractions. The methanol fraction enhanced ALP activity, osteocalcin, integrin-binding sialoprotein, and type I collagen expression, and calcium mineralization. Conversely, the aqueous fraction did not show such effects. Groundnut extract may enhance osteoblast differentiation, and this effect is likely conferred by water insoluble substance(s). |
| Databáze: | OpenAIRE |
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