Assessment of thein vivogenotoxicity of a new formulation ofBacillus thuringiensisvarisraelensisSH-14
| Autor: | Ana M. Bada, Tatiana Molier, C. González, Ailemys Curbelo, A. Mancebo, Graciela Rodríguez, Nidia Rojas, Magdalena Legró, M.E. Arteaga, Nereida Mantilla, Yesenia Rivero, Raiden Grandía, Francisco Contreras, Asnyel F. García, María A. Escalona, Miriam Rosa Lemus |
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| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Toxicological & Environmental Chemistry. 93:691-699 |
| ISSN: | 1029-0486 0277-2248 |
| Popis: | Biological control agents have become a useful alternative for the reduction of the use of chemical insecticides. LABIOFAM (Cuba) is developing a new formulation of a biolarvicide that possesses as active biological agent Bacillus thuringiensis var israelensis serotype H14. In order to evaluate the genotoxicity of this new formulation, an in vivo battery test was used: micronucleus (MN), chromosome aberrations (CAs), and sperm morphology (SM) assays. A dose of 6.45 × 108 spores was administered per animal via oral administration. Bone marrow cells were collected 24 h after a two day treatment for the MN assay, and 24 h after a unique treatment for the CA assay, using cyclophosphamide as the positive control. Sperm cells were collected at 5 weeks from the first of five administrations for the SM test, using acrylamide as positive control. Bacillus thuringiensis var israelensis serotype H14 failed to show either a significative increase of micronucleated polychromatic erythrocytes, chromosomal aberrations, ... |
| Databáze: | OpenAIRE |
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