The role of miRNA in regulating bacterial clearance (P1273)
| Autor: | Hock Tay, Gerard Kaiko, Philip Hansbro, Paul Foster |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 190:56.19-56.19 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.190.supp.56.19 |
| Popis: | RATIONALE MicroRNAs (miRNAs) are small non-coding RNA that can bind to multiple target mRNA to repress protein production. While the expressions of specific miRNAs are known to be dysregulated in multiple human diseases, the role of miRNAs in regulating bacterial infection is unclear. Our aim is to investigate the roles of miRNA in regulating these processes. METHODS BALB/c mice were challenged with low doses of NTHi. Lung miRNAs that were differentially expressed were identified by microarray. miRNA inhibitors (antagomirs) were used to knockdown miRNA expression to investigate the roles of miRNA in bacterial clearance in vitro and in vivo. RESULTS Upon NTHi infection, 20 miRNAs were up-regulated while 52 were down-regulated by > 2.5 fold. Interestingly, knockdown of a specific miRNA in vitro decreased bacterial load and increased phagocytosis by macrophages and neutrophils. Similarly, transfer of macrophages or neutrophils deficient in miRNA into naïve recipient mice leads to enhance clearance of the bacteria in vivo. This enhance clearance is independent of cellular infiltration. Inhibiting miRNA may play a protective role by enhancing the function of innate immune cells during bacterial infection. CONCLUSION Our study suggests that miRNA may play important roles in regulating the innate immune response to bacterial infection. |
| Databáze: | OpenAIRE |
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