DNA-hydrolyzing IgG antibodies from the blood of patients with acquired immune deficiency syndrome

Autor:	Valentina N. Buneva, Baranovskiĭ Ag, M. A. Kharitonova, Siziakina Lp, Nevinskiĭ Ga, Elena S. Odintsova
Rok vydání:	2006
Předmět:	Biophysics Biology Immunoglobulin light chain Molecular biology Immune deficiency syndrome Immunoglobulin G Hydrolysis chemistry.chemical_compound chemistry Biochemistry Structural Biology Polyclonal antibodies DNAase activity biology.protein Antibody DNA
Zdroj:	Molecular Biology. 40:770-777
ISSN:	1608-3245 0026-8933
DOI:	10.1134/s0026893306050128
Popis:	DNAase activity of 110 samples of IgG from the blood of AIDS patients was analyzed. It was shown that the relative activity of preparations varies very much from patient to patient, but 96% preparations show detectable level of DNAase activity. Several rigid criteria were applied and it was shown that DNAase activity is an intrinsic property of antibodies from AIDS patients. It was shown that catalytic activity could posses not only intact IgG, but also separated light chains of polyclonal antibodies. The abzymes catalyze DNA hydrolysis effectively in a wild range of pH (5.0-9.5). K(M) and V(MaKC) values of antibody-dependent hydrolysis of DNA was estimated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6369d9f58de144f4526e975a8633bc9e https://doi.org/10.1134/s0026893306050128 Zobrazit plný text záznamu Full text from SpringerLink