Determination of flomoxef in human plasma by liquid chromatography/electrospray ionization tandem mass spectrometry
| Autor: | Michael Engsig, Natalya I. Vasilevich, Denis N. Kazyulkin, Ekaterina Vlasova, Oxana Yu. Kravtsova, Sergey A. Paramonov |
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| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
Chromatography Formic acid Calibration curve Clinical Biochemistry Extraction (chemistry) Analytical chemistry General Medicine Mass spectrometry Tandem mass spectrometry Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry Ionization Drug Discovery medicine Flomoxef Acetonitrile Molecular Biology medicine.drug |
| Zdroj: | Biomedical Chromatography. 27:1609-1614 |
| ISSN: | 0269-3879 |
| DOI: | 10.1002/bmc.2968 |
| Popis: | A specific, sensitive, rapid and reproducible method for the determination of flomoxef in human plasma using high-performance liquid chromatography–tandem mass spectrometry was developed and validated. Flomoxef was detected using an electrospay ionization method operated in negative-ion mode. Chromatographic separation was performed in gradient elution mode on a Luna® C18(2) column (3 μm, 20 × 4.0 mm) at a flow rate of 1 mL/min and runtime 3.5 min. The mobile phase consisted of acetonitrile and water containing 0.1% formic acid as additive. Extraction of flomoxef from plasma and precipitation of plasma proteins was performed with acetonitrile with an absolute recovery of 86.4 ± 1.6%. The calibration curve was linear with a correlation coefficient of 0.999 over the concentration range 10–5000 ng/mL and the lower limit of quantification was 10 ng/mL. The intra- and inter-day precisions were |
| Databáze: | OpenAIRE |
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