Inhibition of elongation factor 2-dependent translocation by the pokeweed antiviral protein and ricin

Autor:	S.L. Gessner, J.D. Irvin
Rok vydání:	1980
Předmět:	chemistry.chemical_classification Toxin Chromosomal translocation Cell Biology Biology medicine.disease_cause Biochemistry Ribosome Elongation factor chemistry.chemical_compound Enzyme Ricin chemistry medicine Protein biosynthesis Elongation Molecular Biology
Zdroj:	Journal of Biological Chemistry. 255:3251-3253
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(19)85688-0
Popis:	The pokeweed antiviral protein and the toxin ricin are two plant proteins which enzymatically inactivate eukaryotic ribosomes and cause the inhibition of the elongation cycle of protein synthesis. Their mode of inhibition of protein synthesis has been attributed to either the inhibition of enzymatic binding of aminoacyl-tRNA to the acceptor site of the ribosome or its subsequent translocation to the donor site. In this communication, we show that the treatment of ribosomes with either the pokeweed antiviral protein or ricin results in the inhibition of the elongation factor 2-catalyzed translocation of acceptor site aminoacyl-tRNA to the donor site. The inhibitory effects of both proteins are identical and are partially reversed by excess concentrations of elongation factor 2.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::634049ae78ce9d477f45dd8b98d1582d https://doi.org/10.1016/s0021-9258(19)85688-0 Zobrazit plný text záznamu