Poly(ethylene glycol) hydrogel microparticles containing enzyme-fluorophore conjugates for the detection of organophosphorus compounds
| Autor: | Won Gun Koh, Youngsik Lee, Bumsang Kim, Dongkil Choi |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Analyte Chromatography Fluorophore Paraoxon technology industry and agriculture Metals and Alloys macromolecular substances Conjugated system Condensed Matter Physics Fluorescence Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound Photopolymer chemistry PEG ratio Materials Chemistry medicine Organophosphorus compound Organic chemistry Electrical and Electronic Engineering Instrumentation medicine.drug |
| Zdroj: | Sensors and Actuators B: Chemical. 137:209-214 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2008.10.050 |
| Popis: | For the first step in the development of a microanalysis device to detect organophosphorus compounds, PEG hydrogel microparticles containing conjugates of enzyme (AChE) and pH-sensitive fluorophore (SNAFL-1) as an optical biosensor were fabricated by a dispersion photopolymerization. Using the dual emission property of pH-sensitive SNAFL-1, changes in the microenvironment pH due to enzyme-catalyzed reactions with the analyte of interest were determined fluorescently and used to detect the analyte. The activity and sensitivity of the enzyme and pH-sensitive fluorophore were maintained when they were conjugated and encapsulated within the PEG hydrogel microparticles. The fluorescent response of the PEG microparticles containing AChE-SNAFL conjugates was affected by the concentration of a model organophosphorus compound (paraoxon) in the range of 1–20 μmol. These results indicate that the PEG microparticles containing AChE-SNAFL conjugates prepared in this study have a possibility to be used as an optical biosensor to detect organophosphorus compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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