Rapid and economical DNA extraction method from meat matrices containing soybean flour

Autor:	J. Szamos, G. Ujhelyi, É. Gelencsér
Rok vydání:	2011
Předmět:	chemistry.chemical_compound Chromatography chemistry Extraction (chemistry) DNA Solutions Amplicon Biology DNA extraction DNA Food Science
Zdroj:	Acta Alimentaria. 40:426-435
ISSN:	1588-2535 0139-3006
DOI:	10.1556/aalim.2011.0009
Popis:	The main goal of our work was to develop a rapid, simple, and economical DNA extraction method for food (especially for meat products) analysis. This extraction and purification procedure was based on the three-phase partitioning (TPP) method. The developed new DNA-TPP method and Wizard DNA Clean-Up System (Promega, USA) have been compared concerning extraction efficiency, purity and DNA suitability for amplification. The quality and quantity of the purified DNA solutions were controlled by spectrophotometer and the amplification efficiency by simple qualitative PCR. All of prepared DNA solutions were pure enough for the PCR and contained appropriate quantity of DNA. Thus, 118 bp length amplicons could have been obtained by the specific lectin-gene PCR in all cases. This method proved to be an alternative one to isolate DNA from meat samples simply and economically.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::631ab193b4d37e00f664878450ba76db https://doi.org/10.1556/aalim.2011.0009 Zobrazit plný text záznamu