Effect of tobacco tar on Staphylococcus aureus and Candida albicans biofilm formation
| Autor: | Amany Hamouda Mahmoud, Rehab Mahmoud Abd El-Baky, Mamdouh Nabil Samy, Mohamed Kamel, Usama Ramdan Abdel-Mohsen, Amira S. Wanas |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology Chemistry 030106 microbiology Biofilm Plant Science biology.organism_classification Antimicrobial medicine.disease_cause Microbiology Corpus albicans Penicillin 03 medical and health sciences Minimum inhibitory concentration 030104 developmental biology Infectious Diseases Tar (tobacco residue) Staphylococcus aureus medicine Candida albicans medicine.drug |
| Zdroj: | African Journal of Microbiology Research. 11:372-384 |
| ISSN: | 1996-0808 |
| DOI: | 10.5897/ajmr2016.8226 |
| Popis: | Twenty compounds were determined in the tar of cigarette smoke. The tar was obtained using tobacco filters and then analyzed by liquid chromatography-mass spectrometry (LC-MS). Tobacco tar expressed marked decrease in the susceptibility of Staphylococcus aureus to penicillin, tetracycline and amoxicillin/clavulanic antibiotics and showed marked decrease in susceptibility of Candida albicans to the tested antifungals except for clotrimazole and ketoconazole. Tobacco tar decreased the permeability of the tested organisms to ethidium bromide in the presence of antimicrobials, after 2 h for both S. aureus (17.8-20%) and C. albicans (13.3-16.3%) and decreased the adherence of the tested microorganisms at concentrations above Minimal Inhibitory Concentration (MIC). Tobacco tar showed a marked increase in the hydrophobicity of the tested microorganisms by 2.5 to 7 fold. Tobacco tar increased or upregulated ALS1 and HWP1 genes that play an important role in adhesion, hyphae formation and biofilm formation of C. albicans and increased the expression of ica A gene that regulate biofilm formation of S. aureus. Key words: Tobacco tar, Staphylococcus aureus, Candida albicans, antimicrobial susceptibility, ethidium bromide, biofilm formation. |
| Databáze: | OpenAIRE |
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