Biphasic kinetics of âa methanotrophic community is a combination of growth and increased activity per cell
| Autor: | Paul L. E. Bodelier, Marion M. Meima, Miranda Kamst, Anne K. Steenbergh |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
0303 health sciences
Ecology biology 030306 microbiology Methane monooxygenase Kinetics biology.organism_classification Applied Microbiology and Biotechnology Microbiology Methylococcaceae Methane 03 medical and health sciences chemistry.chemical_compound chemistry 13. Climate action Greenhouse gas Environmental chemistry Soil water Anaerobic oxidation of methane biology.protein Bacteria 030304 developmental biology |
| Zdroj: | FEMS Microbiology Ecology. 71:12-22 |
| ISSN: | 1574-6941 0168-6496 |
| DOI: | 10.1111/j.1574-6941.2009.00782.x |
| Popis: | Because methane-oxidizing bacteria (MOB) are the only biological sink for the greenhouse gas methane, knowledge of the functioning of these bacteria in various ecosystems is needed to understand the dynamics observed in global methane emission. The activity of MOB is commonly assessed by methane oxidation assays. The resulting methane depletion curves often follow a biphasic pattern of initial and induced methane oxidation activity, often interpreted as representing the in situ active and total MOB community, respectively. The application of quantitative-PCR on soil incubations, which were stopped before, at and after the transition point in the methane-depletion curve, demonstrated that both pmoA-mRNA was produced as well as substantial cell growth took place already in the initial phase. In addition, type Ia and II MOB displayed markedly different behaviour, which can be interpreted as ecologically different strategies. For the correct interpretation of methane oxidation assays, the use of small time windows is recommended to calculate methane oxidation activities to avoid substantial cell growth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text