46 Effect of guanine substitutions in human telomeric G3(T2AG3)3DNA sequence
| Autor: | Miroslav Fojta, Martin Tomaško, Janos Sagi, Michaela Vorlíčková |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Journal of Biomolecular Structure and Dynamics. 31:28-28 |
| ISSN: | 1538-0254 0739-1102 |
| DOI: | 10.1080/07391102.2013.786479 |
| Popis: | In addition to the well-known Watson–Crick double helix, DNA can form other structures. One of them is a four-stranded quadruplex, formation of which was also acknowledged in in vivo conditions. It was suggested that the presence of quadruplexes in e.g. telomeric region has a significant biological importance. We have studied structural properties of the human telomeric quadruplex formed by G3(T2AG3)3 and related sequences, in which each guanine base was one-by-one replaced by adenine. In the next step, we have studied sequences, in which two, or even four guanines were replaced by adenine. These sequences were studied in the presence of sodium or potassium ions. Using CD spectroscopy, UV thermal stability measurements, and polyacrylamide gel electrophoresis we found that none of the substitutions hindered the formation of the antiparallel quadruplex formed by the unsubstituted sequence in sodium solutions. However, the effect of substitution differed depending on the position of the guanine replaced. The... |
| Databáze: | OpenAIRE |
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