CHITOSAN/CARRAGEENAN/LYSOZYME PARTICLES: SYNTHESIS, CHARACTERIZATION AND ANTIFUNGAL ACTIVITY AGAINST Aspergillus parasiticus
| Autor: | Cynthia Nazareth Hernández-Téllez, Mario Onofre Cortez-Rocha, W. Torres-Arreola, A. Burgos Hernández, María Guadalupe Burboa-Zazueta, Maribel Plascencia-Jatomea, Ema Carina Rosas-Burgos, Jaime Lizardi-Mendoza |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology General Chemical Engineering 010501 environmental sciences Actin cytoskeleton biology.organism_classification 01 natural sciences Aspergillus parasiticus Carrageenan Chitosan 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Dynamic light scattering Fluorescence microscope Biophysics Lysozyme Cytotoxicity 0105 earth and related environmental sciences |
| Zdroj: | Revista Mexicana de Ingeniería Química. 17:897-912 |
| ISSN: | 2395-8472 1665-2738 |
| Popis: | In this study, particles of chitosan-carrageenan (Cs-Crg) and chitosan-carrageenan-lysozyme (CS-Crg-LZ) were synthesized by ionic complexation. The particles were analyzed by atomic force microscopy, dynamic light scattering and FT-IR, and the percentage of association of lysozyme was determined. The biological activity of the particles was evaluated by viability assays of Aspergillus parasiticus spores and cytotoxicity in ARPE-19 cells. The effect on subcellular structures was analyzed by fluorescence microscopy. The addition of lysozyme reduced the size of the particles. The size and Z potential of the synthesized particles were 725±5.5 and 250±8.1 nm and 28.7±1.0 and 21.6±0.8 mV for CS-Crg and CS-Crg-LZ, respectively. CS-Crg-LZ and CS-Crg particles exhibited a higher (p |
| Databáze: | OpenAIRE |
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