| Autor: |
Andrew J. Thompson, Ronald R.D. Crop, J. R. Ellis, N. Harris, I. Marta Evans, John A. Gatehouse, Angray S. Kang, Donald Boulter, Jenny N. Yarwood, A. H. Shirsat |
| Rok vydání: |
1988 |
| Předmět: |
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| Zdroj: |
Biochemie und Physiologie der Pflanzen. 183:183-197 |
| ISSN: |
0015-3796 |
| DOI: |
10.1016/s0015-3796(88)80098-2 |
| Popis: |
Summary and Introduction The pea legumin seed storage proteins represent one of the most highly characterised group of proteins and genes in plants. The gene family consists of at least two classes of legumin genes which encode distinct but related polypeptides. In the homologous host ( Pisum sativum L.) these genes are expressed to different degrees as judged by different levels of the proteins accumulated, steady-state messenger RNAs and nuclear transcription rates. Such experiments indicate that the control of gene expression is primarily at the transcriptional level, probably within the non-coding flanking gene sequences; however, post-transcriptional controls operate to modify expression at the translational level. A major, active, pea legumin gene has been transferreed intact to Nicotiana and is expressed in the transgenic plants. The expression of this construct has been shown to retain its organ specificity, and pea legumin protein is accumulated in the Nicotiana seeds. An assessment of the levels and the sites of deposition of the legumin protein synthesised indicate that not only are the gene control sequences present and functioning in the heterologous host but the amino acid sequences which determine targeting and processing of the newly synthesised protein are also recognised in Nicotiana . Similarly such targeting sequences when synthesized in yeast also direct the legumin protein to membrane compartments. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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