Lagerstroemia ovalifolia Exerts Anti- Inflammatory Effects in Mice of LPSInduced ALI via Downregulating of MAPK and NF-κB Activation
| Autor: | Ngatinem, Seong-Man Kim, Ji-Won Park, Nam Hoon Kwon, Jin-Hyub Paik, Sri Ningsih, Sang-Bae Han, Jae-Hong Min, Kyung-Seop Ahn, Sei-Ryang Oh, Sangho Choi, Soo Hyeon Goo, Jae-Won Lee |
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| Rok vydání: | 2021 |
| Předmět: |
MAPK/ERK pathway
Lipopolysaccharide biology medicine.drug_class Monocyte NF-κB General Medicine respiratory system Pharmacology Lung injury Applied Microbiology and Biotechnology Anti-inflammatory respiratory tract diseases Proinflammatory cytokine Nitric oxide synthase chemistry.chemical_compound medicine.anatomical_structure chemistry biology.protein medicine Biotechnology |
| Zdroj: | Journal of Microbiology and Biotechnology. 31:1501-1507 |
| ISSN: | 1738-8872 1017-7825 |
| DOI: | 10.4014/jmb.2107.07023 |
| Popis: | Lagerstroemia ovalifolia Teijsm. & Binn. (LO) (crape myrtle) has reportedly been used as traditional herbal medicine (THM) in Java, Indonesia. Our previous study revealed that the LO leaf extract (LOLE) exerted anti-inflammatory effects on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Based on this finding, the current study aimed to evaluate the protective effects of LOLE in a mouse model of LPS-induced acute lung injury (ALI). The results showed that treatment with LPS enhanced the inflammatory cell influx into the lungs and increased the number of macrophages and the secretion of the inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) of mice. However, these effects were notably abrogated with LOLE pretreatment. Furthermore, the increase of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and monocyte chemoattractant protein-1 (MCP-1) expression in the lung tissues of mice with ALI was also reversed by LOLE. In addition, LOLE significantly suppressed the LPS-induced activation of the MAPK/NF-κB signaling pathway and led to heme oxygenase-1 (HO-1) induction in the lungs. Additionally, in vitro experiments showed that LOLE enhanced the expression of HO-1 in RAW264.7 macrophages. The aforementioned findings collectively indicate that LOLE exerts an ameliorative effect on inflammatory response in the airway of ALI mice. |
| Databáze: | OpenAIRE |
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