Use of the fluorescence technique as an absolute method for obtaining mean relaxation times of globular proteins

Autor:	A. J. McAlister, R. F. Steiner
Rok vydání:	1957
Předmět:	chemistry.chemical_classification Residue (complex analysis) biology Globular protein Relaxation (NMR) Analytical chemistry Fluorescence chemistry.chemical_compound Nuclear magnetic resonance chemistry Excited state biology.protein Degree of polarization Bovine serum albumin Lysozyme
Zdroj:	Journal of Polymer Science. 24:105-123
ISSN:	1542-6238 0022-3832
DOI:	10.1002/pol.1957.1202410513
Popis:	Measurements of excited lifetime and of degree of polarization have been combined to yield values for the mean relaxation time of fluorescent conjugates of bovine serum albumin, egg albumin, and lysozyme. Evidence has been presented for the validity of relaxation times thus computed and for the absence of contributions from nonrandomness of fluorescent residue orientation and from internal degrees of rotational freedom.
Databáze:	OpenAIRE
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