Immobilization of Invertase in Copolymer of 2,5-Di(thiophen-2-yl)-1-p-Tolyl-1H-Pyrrole with Pyrrole

Autor:	Başak Yiĝitsoy, Selin Celebi, Esin Ibibikcan, Levent Toppare, Senem Kiralp Kayahan
Rok vydání:	2009
Předmět:	Conductive polymer Polymers and Plastics Immobilized enzyme biology Chemistry Enzyme electrode General Chemistry Enzyme assay chemistry.chemical_compound Invertase Monomer Polymer chemistry Materials Chemistry Ceramics and Composites Copolymer biology.protein Pyrrole
Zdroj:	Journal of Macromolecular Science, Part A. 46:739-744
ISSN:	1520-5738 1060-1325
DOI:	10.1080/10601320903004434
Popis:	Immobilization of invertase in conducting copolymer matrix of 2,5-di(thiophen-2-yl)-1-p-tolyl-1H-pyrrole with pyrrole (poly(DDTP-co-Py)) was achieved via electrochemical polymerization. Kinetic parameters, Michaelis-Menten constant, Km and the maximum reaction rate, Vmax were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined. Immobilized invertase reveals maximum activity at 50°C and; pH 8 and pH 4 for two copolymer matrices. Although the same two monomers are utilized for the copolymer synthesis, the way the copolymer is produced results in quite different responses in terms of enzyme activity, optimum pH and kinetic parameters. Excellent operational stability of the enzyme electrodes enables their repetitive use in the determination of invert sugar.
Databáze:	OpenAIRE
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