Luminescence determination of DNA using terbium complexes with 2-oxo-4-hydroxyquinoline-3-carboxylic acid amides as probes

Autor:	V. P. Antonovich, A. V. Egorova, Yu. V. Skripinets, Igor V. Ukrainets
Rok vydání:	2006
Předmět:	chemistry.chemical_classification Detection limit Chemistry Carboxylic acid Inorganic chemistry chemistry.chemical_element Terbium Analytical Chemistry Ion chemistry.chemical_compound Amide Molecule Luminescence DNA
Zdroj:	Journal of Analytical Chemistry. 61:44-51
ISSN:	1608-3199 1061-9348
DOI:	10.1134/s1061934806010102
Popis:	It was found that the presence of DNA significantly enhanced the 4f luminescence of terbium(III) in its complexes with a number of 2-oxo-4-hydroxyquinoline-3-carboxylic acid amide derivatives (L1-L6). For the high-sensitivity determination of DNA, L1 and L4 were chosen from two proposed groups, in complexes with which terbium ions exhibited the most intense luminescence. Under optimum conditions, the luminescence intensity of terbium was proportional to the concentration of DNA over the ranges 0.025–1.2 μg/mL (detection limit of 10 ng/mL) and 0.01–1.2 λg/mL (detection limit of 3 ng/mL) for L1 and L4, respectively. The mechanisms of the interaction of Tb-L complexes with DNA molecules were hypothesized.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::62a86960c98b68aa13ed3361fc2b1521 https://doi.org/10.1134/s1061934806010102 Zobrazit plný text záznamu Plný text ve formátu PDF