| Autor: |
Wolfgang Michel, Helmut Determann |
| Rok vydání: |
1966 |
| Předmět: |
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| Zdroj: |
Journal of Chromatography A. 25:303-313 |
| ISSN: |
0021-9673 |
| DOI: |
10.1016/s0021-9673(01)98246-9 |
| Popis: |
Nearly thirty papers in the literature, concerning the chromatographic behaviour of macromolecules on columns of porous gels demonstrate a linear relation between the elution volume and the logarithm of the molecular weight. The current theoretical treatments of gel chromatography do not explain this relationship. Data from ten recent papers were behaviour of globular proteins on Sephadex gels can be described by the special equations: A mathematical treatment of the gel chromatography process, based on these empirical equations and avoiding assumptions about the microstructure of the gel, strengthens the concept of the exclusion mechanism. It results in the general equation: covering the field of very porous gels (Sephadex G-75 to G-200). From experiments with globular proteins on colums of any gel their molecular weight may be determined, if—besides the void volume (Vo)—the wet density of the gel (d) and the elution pattern of only one test protein is known. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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