Electronic structure study of the bases in DNA duplexes by in situ photon-in/photon-out soft X-ray spectroscopy
| Autor: | J. M. Griffiss, Per-Anders Glans, Kevin E. Smith, Johan Söderström, T. Learmonth, Way-Faung Pong, Yi Luo, J.-H. Guo, Joseph Nordgren, J.W. Chiou, H. Cheng, M. West, S. Kastanov |
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| Rok vydání: | 2010 |
| Předmět: |
Quantitative Biology::Biomolecules
X-ray absorption spectroscopy Radiation Chemistry Astrophysics::High Energy Astrophysical Phenomena Fermi level Electronic structure Condensed Matter Physics Atomic and Molecular Physics and Optics Spectral line Electronic Optical and Magnetic Materials symbols.namesake Chemical bond Chemical physics Excited state symbols Emission spectrum Physical and Theoretical Chemistry Atomic physics Spectroscopy |
| Zdroj: | Journal of Electron Spectroscopy and Related Phenomena. 181:197-201 |
| ISSN: | 0368-2048 |
| DOI: | 10.1016/j.elspec.2010.05.014 |
| Popis: | Understanding protein functionality is of fundamental importance in biochemistry. Soft X-ray transitions, where the core-level vacancies are filled by the valence-orbital electrons, give direct information about the chemical bonding. Soft X-ray absorption and emission study of poly(dG) -poly(dC) in aqueous solutions can elucidate the relation between the structure and functionality of proteins. We report the N K-edge soft X-ray absorption (XAS) and resonant soft X-ray emission spectroscopy (XES) to characterize the electronic structure near the Fermi level of DNA duplexes to specify the charge migration mechanism. Since N atoms are included in only bases in DNA duplexes, the XES spectra excited from N 1s to unoccupied states purely extract the electronic orbital features of the bases in DNA. The fact that N atoms in different bonding environments form well-defined structure has been determined. The experimental findings provide the evidence for the charge-hopping and/or charge-transfer effects in understanding of electric conduction in DNA duplexes when electrons pass through the π* states of DNA bases. |
| Databáze: | OpenAIRE |
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