Sesamol-Loaded PLGA Nanosuspension for Accelerating Wound Healing in Diabetic Foot Ulcer in Rats
| Autor: | Karthik Gourishetti, Nitesh Kumar, Srinivas Reddy Jitta, Pawan G. Nayak, Nandakumar Krishnadas, Rekha R Shenoy, Lalit Kumar, Navya Ajitkumar Bhaskaran, Raghuvir Keni |
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| Rok vydání: | 2020 |
| Předmět: |
Biophysics
Pharmaceutical Science Bioengineering 02 engineering and technology Pharmacology 010402 general chemistry 01 natural sciences Fibroblast migration Biomaterials Masson's trichrome stain chemistry.chemical_compound In vivo Drug Discovery medicine Sesamol business.industry Organic Chemistry General Medicine 021001 nanoscience & nanotechnology medicine.disease Diabetic foot 0104 chemical sciences PLGA Diabetic foot ulcer chemistry 0210 nano-technology business Wound healing |
| Zdroj: | International Journal of Nanomedicine. 15:9265-9282 |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s268941 |
| Popis: | Background: Diabetic foot ulcer is an intractable complication of diabetes, characterized by the disturbed inflammatory and proliferative phases of wound healing. Sesamol, a phenolic compound, has been known for its powerful antioxidant, anti-inflammatory, antihyperglycaemic and wound healing properties. The aim of the present study was to develop a sesamol nano formulation and to study its effect on the various phases of the wound healing process in diabetic foot condition.Methods: Sesamol-PLGA (SM-PLGA) nanosuspension was developed using nanoprecipitation method. TEM, in vitro drug release assay and in vivo pharmacokinetic studies were performed for the optimised formulation. Diabetic foot ulcer (DFU) in high fat diet (HFD)-fed streptozotocin-induced type-II diabetic animal model was used to assess the SM- PLGA nanosuspension efficacy. SM-PLGA nanosuspension was administered by oral route. TNF-α levels were estimated using ELISA and Western blot analysis was performed to assess the effect on the expression of HSP-27, ERK, PDGF-B and VEGF in wound tissue. Wound reepithelization,fibroblast migration, collagen deposition and inflammatory cell infiltration were assessed by H&E and Masson’s trichrome staining. Effect on angiogenesis was assessed by CD-31 IHC staining in wound sections.Results: The optimized SM-PLGA nanosuspension had an average particle size of |
| Databáze: | OpenAIRE |
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