Optimization of flavonoids extraction from Lippia graveolens and Lippia origanoides chemotypes with ethanol-modified supercritical CO2 after steam distillation
| Autor: | Elena E. Stashenko, José M. del Valle, Yuri Córdoba, Jairo René Martínez, Jesica Julieth Mejía, Julián Andrés Ariza Arias |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Supercritical carbon dioxide Chromatography biology Chemotype 010405 organic chemistry Chemistry Extraction (chemistry) biology.organism_classification 01 natural sciences Supercritical fluid 0104 chemical sciences law.invention Steam distillation chemistry.chemical_compound Lippia graveolens law Oleoresin Agronomy and Crop Science Essential oil 010606 plant biology & botany |
| Zdroj: | Industrial Crops and Products. 146:112170 |
| ISSN: | 0926-6690 |
| Popis: | Steam distillation of essential oils from Lippia origanoides K. and Lippia graveolens K. leaves plant material residues that can be valorized. Flavonoids can be selectively extracted from these substrates using ethanol-modified supercritical CO2, as attempted in this work. The thymol-rich L. origanoides chemotype was used to test extraction reproducibility. The effects of pressure, temperature, time, CO2 mass flow, particle size, and percent ethanol on the extraction yield of oleoresin and flavonoids were examined with a 2(6−2) fractional factorial screening design. The variables with a significant influence on yields (pressure, time, CO2 mass flow, and percent ethanol) were used in a simplex optimization to determine the conditions for maximum extraction from L. graveolens and three L. origanoides chemotypes. The extracts’ flavonoid content was estimated by UV–vis spectroscopy with a multivariate calibration model. UHPLC-ESI(+)-Orbitrap-MS was employed for extract identification and quantification. The extracts from the various plant material residues had qualitative and quantitative differences in flavonoid composition. Under the best extraction conditions found (307 bar, 5 % ethanol, 43 g CO2/min), the steam-distilled plant material from the phellandrene-rich L. origanoides chemotype afforded 4.1 g of pinocembrin per kg of dry substrate. Results of this work facilitate the implementation of supercritical CO2 extraction processes to valorize byproducts of the essential oil industry by obtaining extracts that contain flavonoids. |
| Databáze: | OpenAIRE |
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