Miltefosine and polyhexamethylene biguanide: a new drug combination for the treatment ofAcanthamoebakeratitis

Autor: Andreas Obwaller, Ayse Vural, Ayhan Dursun, Zubeyde Akin Polat, Julia Walochnik, Mustafa Arici
Rok vydání: 2013
Předmět:
Zdroj: Clinical & Experimental Ophthalmology. 42:151-158
ISSN: 1442-6404
DOI: 10.1111/ceo.12120
Popis: Background In this study, a series of compounds – miltefosine, polyhexamethylene biguanide, chlorhexidine and propamidine isethionate – and combinations of the latter three agents with miltefosine were prepared and used in a rat model for the topical treatment of Acanthamoeba keratitis. Methods The corneas of rats were infected with Acanthamoeba hatchetti. On the fifth day, all corneas were microscopically examined in order to determine the grade of infections. Nine groups were then prepared: miltefosine (65.12 μg/mL); chlorhexidine (0.02%); polyhexamethylene biguanide (0.02%), propamidine isethionate (0.1%), miltefosine plus chlorhexidine, miltefosine plus polyhexamethylene biguanide; miltefosine plus propamidine isethionate; infected control; and a non-infected control group. The treatment was continued for 28 days. After the treatment, the corneas were excised and used for Acanthamoeba culture to investigate the presence of Acanthamoeba growth. For the determination of cytotoxicity of the drugs on L929 cells, colorimetric assays were performed. Results The best treatment results were obtained from the polyhexamethylene biguanide plus miltefosine group; the ratio of fully recovered eyes was 28.4%. It was proven that the miltefosine–polyhexamethylene biguanide combination yielded the highest anti-acanthamoebal activity in that approximately 86% of the eyes were cleared from amoebae. The cytotoxicity values of the miltefosine and the control groups were compared with other groups and found to be statistically different (P
Databáze: OpenAIRE