Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR
| Autor: | Zion Dar, Menachem Bornstein, Victor Gaba, Mouhammad Zeidan, Amit Gal-On, Malenia Pearlsman, Liat Avrahami-Moyal, Marina Brumin, Yehudit Tam, Shanmugam Prakash, Diana Leibman, Noa Sela, Uri Zig |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology business.industry fungi food and beverages Sowing Plant Science Solanum tuberosum biology.organism_classification 01 natural sciences Biotechnology Crop 03 medical and health sciences Horticulture 030104 developmental biology Real-time polymerase chain reaction Potato virus Y Insect Science Plant virus TaqMan Cultivar business 010606 plant biology & botany |
| Zdroj: | Phytoparasitica. 45:591-598 |
| ISSN: | 1876-7184 0334-2123 |
| DOI: | 10.1007/s12600-017-0612-z |
| Popis: | Potato (Solanum tuberosum) is the largest crop in Israel. Production is based on the import of seed tubers from Europe for the spring crop. Imported tubers are generally free from virus infection. The most important virus infecting potato is Potato virus Y (PVY), which may cause severe damage to marketable yields. In Israel, tubers from the spring harvest are stored over the summer for planting in the autumn. It is important to be able to determine the infection rate of seed tuber lots from the spring harvest prior to storage. Commonly, infection is measured by sprouting tubers and measuring virus titre in the leaves using ELISA (the “Growing-On test”), which takes at least 6 weeks to give results. There is a need for a faster method to produce results, such as Taqman Real Time PCR (qPCR), for direct analysis of viral infection in tubers at harvest. To use qPCR as a diagnostic tool, it is necessary to demonstrate that both techniques give comparable results on batches of field-grown tubers. Such a comparison was performed on potential seed tuber lots of 14 different cultivars over three Israeli spring harvests (2013–2015). The agreement between the results of the two techniques was not of high statistical significance. However, the qPCR technique can distinguish well, by binary classification, between tuber lots with a low PVY infection rate ( |
| Databáze: | OpenAIRE |
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