Monitoring Structural Changes of Proteins in an Ion Trap over ∼10−200 ms: Unfolding Transitions in Cytochrome c Ions

Autor:	Ethan R. Badman, David E. Clemmer, Cherokee S. Hoaglund-Hyzer
Rok vydání:	2001
Předmět:	Quantitative Biology::Biomolecules biology Chemistry Cytochrome c Analytical chemistry Mass spectrometry Analytical Chemistry Ion Trap (computing) Physics::Plasma Physics biology.protein Ion trap Extended time Conformational isomerism
Zdroj:	Analytical Chemistry. 73:6000-6007
ISSN:	1520-6882 0003-2700
Popis:	A new technique for studying the time dependence of conformational changes of gas-phase protein ions is described. In this approach, a short pulse of electrosprayed protein ions is introduced into an ion trap and stored. After a defined time period, the distribution of ions is ejected from the trap into an ion mobility/time-of-flight mass spectrometer. Combined measurements of mobilities and flight times in the mass spectrometer provide information about the abundances of different conformer types and charge-state distributions. By varying the storage time in the trap, it is possible to monitor changes in ion conformation that occur over extended time periods (∼10−200 ms). The method is demonstrated by examining changes in cytochrome c ion conformations for the +7 to +10 charge states.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6257be52d6b93926892d79504da07ac4 https://doi.org/10.1021/ac010744a Zobrazit plný text záznamu