| Popis: |
Objective: To observe the behavioral changes of aged rats with hypercholesterolemia after inhalation of 1.0 MAC and 1.3MAC sevoflurane , the levels of hippocampal ApoE3, ApoE4 and Aβ1-42, as well as the changes of Aβ1-42 levels of optical density in hippocampal CA3 and CA1 regions, and to investigate the effects of different concentrations of sevoflurane on cognitive function in aged rats with hypercholesterolemia.Method: The 15-month-old male SD rats were fed a high-fat diet for 9 months. Rats successfully modeled (N=54) were randomly divided into three groups:control group (Con group, n=18), low concentration sevoflurane group ( Sev1.0 group, n=18), high concentration sevoflurane group (Sev1.3 group, n=18). Rats in the three groups inhaled aero mixed gas(1L/min O2+1L/min Air), 1.0MAC sevoflurane and 1.3MAC sevoflurane for 2h respectively.1 day, 30 days and 90 days after sevoflurane treatment were defined as T1, T30 and T90 experimental periods, respectively. In the three experiments, 6 rats were randomly selected from Con group, Sev1.0 group and Sev1.3 group to complete the behavior experiment in Morris water maze.Subsequently, the levels of ApoE3, ApoE4 and Aβ1-42 in the left hippocampus were detected by Western Blot.Expression of Aβ1-42 in the right hippocampal CA3 and CA1 regions of rats in each group was detected by frozen immunofluorescence assay.Result: 1. No behavioral changes were found in T1, T30 and T90 experiments.2. At T1 and T30, ApoE4 and Aβ1-42 in Sev1.3 group was significantly different from that in Con group. At T90, there was no difference in the levels of ApoE4 and Aβ1-42 between groups. ApoE3 expression was not statistically significant in the three experimental periods. 3. At T1 and T30, the average optical density of Aβ1-42 in CA3 and CA1 region, Sev1.3 group was significantly different from that in Con group. At T90, there was no significant difference between groups.Conclusion: After inhalation of 1.3MAC sevoflurane in aged SD hypercholesterolemia rats, the levels of ApoE4 and Aβ1-42 were increased in hippocampus at early and middle stage, and the average optical density of Aβ1-42 was increased in CA3 and CA1 area. The change trend of the two ones was consistent, but not enough to cause behavioral changes. |
