Complete genome sequence of Aphid lethal paralysis virus from metagenomic analysis of Cestrum elegans small RNAs
| Autor: | Shifang Li, Vahid Roumi, Mona Gazel, Kadriye Çağlayan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Dicistroviridae Genetics Whole genome sequencing Cestrum elegans biology food and beverages Cripavirus biology.organism_classification Genome 03 medical and health sciences Complete sequence 030104 developmental biology 0302 clinical medicine Intergenic region Aphis nerii 030220 oncology & carcinogenesis |
| Zdroj: | Gene Reports. 18:100566 |
| ISSN: | 2452-0144 |
| DOI: | 10.1016/j.genrep.2019.100566 |
| Popis: | Passive “vector” plants can harbor insect viruses without supporting their replication and may have epidemiological consequences. We report complete sequence of Aphid lethal paralysis virus (ALPV, Cripavirus, Dicistroviridae) from small RNA sequencing of a new passive “vector” plant, Cestrum elegans in Turkey using de novo assembly and annotating of contigs. Its genome is 9837 nt in length which encodes for 2 ORFs (including an intergenic untranslated region (IGR) of 196 nt) flanked by a 525 nt 5′ UTR and a 596 nt 3′ UTR. Multiple alignment and phylogenetic analysis showed that the isolate shares highest identity with ALPV-An (JX480861) isolated from oleander aphid, Aphis nerii and was placed into the same clade with other isolates of passive “vector” plants comprising cucumber and maize isolates. |
| Databáze: | OpenAIRE |
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