| Autor: |
Luis A. Justulin, Ary Fernandes Júnior, Carlos Alberto Labate, Arnold J. M. Driessen, Jeroen G. Nijland, Mônica Teresa Veneziano Labate, Erica Ramos, Marjorie de Assis Golim, Matheus Naia Fioretto, Fabiana Barcelos Furtado, Camila Cristina de Oliveira Alves, Luiz Henrique Cardoso, Cesar Martins, Rodrigo de Oliveira Almeida, Lucas Cardoso Lazari, Lauana Fogaça de Almeida, Thais Regiani Cataldi, Rejane Maria Tommasini Grotto, Guilherme Targino Valente, Rafael Luiz Buogo Coan, Ivan Rodrigo Wolf, Rafael T. Nakajima, Lucas Farinazzo Marques, Camila Moreira Pinto, Rafael Plana Simōes, Amanda Piveta Schnepper, Leonardo Nazario de Moraes |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.08.04.455136 |
| Popis: |
Ethanol (EtOH) is a substantial stressor for Saccharomyces cerevisiae. Data integration from strains with different phenotypes, including EtOH stress-responsive lncRNAs, are still not available. We covered these issues seeking systems modifications that drive the divergences between higher (HT) and lower (LT) EtOH tolerant strains under their highest stress conditions. We showed that these phenotypes are neither related to high viability nor faster population rebound after stress relief. LncRNAs work on many stress-responsive systems in a strain-specific manner promoting the EtOH tolerance. Cells use membraneless RNA/protein storage and degradation systems to endure the stress harming, and lncRNAs jointly promote EtOH tolerance. CTA1 and longevity are primer systems promoting phenotype-specific gene expression. The lower cell viability and growth under stress is a byproduct of sphingolipids and inositol phosphorylceramide dampening, acerbated in HTs by sphinganine, ERG9, and squalene overloads; LTs diminish this harm by accumulating inositol 1-phosphate. The diauxic shift drives an EtOH buffering by promoting an energy burst under stress, mainly in HTs. Analysis of mutants showed genes and lncRNAs in three strains critical for their EtOH tolerance. Finally, longevity, peroxisome, energy and lipid metabolisms, RNA/protein degradation and storage systems are the main pathways driving the EtOH tolerance phenotypes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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