Development of low-cost serological immunoassay to detect antiviral antibodies to Sars-Cov-2 Spike protein

Autor:	Marcio C. Bajgelman, Hui Lin-Wang, Jessica Bassani, Carlos Gun, Camila Canateli, Gabriel C. Lima, Elaine Cardoso, Jessica M. Toscaro, Ana Carolina Pagliarone, Cely Saad Abboud, Luis A. Peroni, R. L. Viana, Kleber G. Franchini
Rok vydání:	2021
Předmět:	chemistry.chemical_classification medicine.diagnostic_test Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) fungi Biology Virology Serology Antigen chemistry Immunoassay medicine biology.protein Spike (software development) Seroconversion Antibody Glycoprotein
Popis:	Seroconversion to SARS-CoV-2 has been widely studied to evaluate infection spreading for epidemiological purpose, or even in studies of protective immunity in convalescent or vaccinated individuals. The viral particle has an envelope harboring the spike glycoprotein, which can be used as an antigen for assay development, to detect antiviral antibodies to SARS-CoV-2. Since several vaccines encode a spike subunit, the full length spike-based immunoassay should be a universal tool to evaluate seroconversion. In this manuscript, we propose a low-cost ELISA that can be used to detect antiviral IgG to SARS-CoV-2 in human serum.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::620d5f3a3e43919d6a92580bbe97b48e https://doi.org/10.1101/2021.06.07.21258114 Zobrazit plný text záznamu