The clinical significance of PNH-phenotype cells accounting for < 0.01% of total granulocytes detected by the Clinical and Laboratory Standards Institute methods in patients with bone marrow failure

Autor: Kohei Hosokawa, Ken Ishiyama, Haruhiko Ninomiya, Yuji Yonemura, Chiharu Sugimori, Mai Anh Thi Nguyen, Yukari Shirasugi, Toshiyuki Ikemoto, Yoshihiko Nakamura, Tsutomu Shichishima, Shinji Nakao, Shigeru Chiba, Yasutaka Ueda, Junichi Nishimura, Yuzuru Kanakura, Tatsuya Kawaguchi, Hideyoshi Noji, Naoshi Obara, Kiyoshi Ando
Rok vydání: 2020
Předmět:
Zdroj: Annals of Hematology. 100:1975-1982
ISSN: 1432-0584
0939-5555
DOI: 10.1007/s00277-020-04314-w
Popis: Small populations of glycosylphosphatidylinositol-anchored protein-deficient (GPI[-]) cells accounting for up to 0.01% of total granulocytes can be accurately detected by a high-sensitivity flow cytometry (FCM) assay established by the Clinical and Laboratory Standards Institute (CLSI method) and have a prognostic value in bone marrow failure (BMF); however, the significance of GPI(-) granulocytes accounting for 0.001-0.009% of granulocytes remains unclear. To clarify this issue, we examined the peripheral blood of 21 BMF patients in whom minor (around 0.01%) populations of GPI(-) granulocytes had been previously detected by a different high-resolution FCM method (OPTIMA method, which defines ≥ 0.003% GPI(-) granulocytes as an abnormal increase) using both the CLSI and OPTIMA methods simultaneously. These two methods detected an "abnormal increase" in GPI(-) granulocytes in 10 patients (48%) and 17 patients (81%), respectively. CLSI detected 0.002-0.005% (median, 0.004%) GPI(-) granulocytes in 7 patients who were deemed positive for PNH-type cells according to the OPTIMA method, which detected 0.003-0.012% (median 0.006%) GPI(-) granulocytes. The clone sizes of GPI(-) cells detected by each assay were positively correlated (r = 0.994, p < 0.001). Of the seven patients who were judged positive for PNH-type cells by OPTIMA alone, five received immunosuppressive therapy, and all of them achieved a partial or complete response. GPI(-) granulocytes detected in BMF patients by the CLSI method should thus be considered significant, even at percentages of < 0.01%.
Databáze: OpenAIRE
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