Preparation of Amplicon Libraries for Metabarcoding of Marine Eukaryotes Using Illumina MiSeq: The Dual-PCR Method
| Autor: | Sarah J. Bourlat, Quiterie Haenel, Matthieu Leray, Jennie Finnman |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics Illumina miseq Computational biology Small ribosomal subunit Amplicon Biology 010603 evolutionary biology 01 natural sciences law.invention 03 medical and health sciences 030104 developmental biology law Mitochondrial cytochrome Pcr method Polymerase chain reaction |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493937721 |
| Popis: | This protocol details the preparation of multiplexed amplicon libraries for metabarcoding (amplicon-based) studies of microscopic marine eukaryotes. Metabarcoding studies, based on the amplification of a taxonomically informative marker from a collection of organisms or an environmental sample, can be performed to analyze biodiversity patterns or predator-prey interactions. For Metazoa, we use the mitochondrial cytochrome oxidase 1 (CO1) or the small ribosomal subunit (SSU) markers. Here, we describe a strategy for the preparation of multiplexed Illumina MiSeq libraries using a dual-PCR approach for the addition of index and adaptor sequences. |
| Databáze: | OpenAIRE |
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