Insight into Notch Signaling Steps That Involve pecanex from Dominant-Modifier Screens in Drosophila
| Autor: | Yu Atsumi, Ami Yamagishi, Kenji Matsuno, Izumi Morita, Tomoko Yamakawa, Shiori Kubo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Genetics Mesoderm animal structures Neuroectoderm biology Notch signaling pathway biology.organism_classification Transmembrane protein 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Neuroblast medicine Drosophila melanogaster Enhancer Transcription factor |
| Zdroj: | Genetics. 209:1099-1119 |
| ISSN: | 1943-2631 |
| Popis: | Notch signaling plays crucial roles in intercellular communications. In Drosophila, the pecanex (pcx) gene, which encodes an evolutionarily conserved multi-pass transmembrane protein, appears to be required to activate Notch signaling in some contexts, especially during neuroblast segregation in the neuroectoderm. Although Pcx has been suggested to contribute to endoplasmic reticulum homeostasis, its functions remain unknown. Here, to elucidate these roles, we performed genetic modifier screens of pcx. We found that pcx heterozygotes lacking its maternal contribution exhibit cold-sensitive lethality, which is attributed to a reduction in Notch signaling at decreased temperatures. Using sets of deletions that uncover most of the second and third chromosomes, we identified four enhancers and two suppressors of the pcx cold-sensitive lethality. Among these, five genes encode known Notch-signaling components: big brain, Delta (Dl), neuralized (neur), Brother of Bearded A (BobA), a member of the Bearded (Brd) family, and N-ethylmaleimide-sensitive factor 2 (Nsf2). We showed that BobA suppresses Dl endocytosis during neuroblast segregation in the neuroectoderm, as Brd family genes reportedly do in the mesoderm for mesectoderm specification. Analyses of Nsf2, a key regulator of vesicular fusion, suggested a novel role in neuroblast segregation, which is distinct from Nsf2’s previously reported role in imaginal tissues. Finally, jim lovell, which encodes a potential transcription factor, may play a role in Notch signaling during neuroblast segregation. These results reveal new research avenues for Pcx functions and Notch signaling. |
| Databáze: | OpenAIRE |
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