| Autor: | Fernando Albericio, Guillermo J. Albanesi, Marina Etcheverrigaray, Silvia A. Camperi, M. Oggero Eberhardt, Nancy B. Iannucci, Osvaldo Cascone, A. Messeguer |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
Chromatography Tetrapeptide biology Ligand medicine.drug_class Bioengineering Peptide General Medicine Monoclonal antibody Applied Microbiology and Biotechnology chemistry.chemical_compound chemistry Affinity chromatography medicine biology.protein Agarose Antibody Peptide library Biotechnology |
| Zdroj: | Biotechnology Letters. 25:1545-1548 |
| ISSN: | 0141-5492 |
| DOI: | 10.1023/a:1025464918453 |
| Popis: | The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix obtained by attachment of APAR to agarose, having a peptide density of 0.5 μmol ml−1, showed a maximum capacity of 9.1 mg Mab ml−1 and a dynamic capacity of 3.9 mg Mab ml−1. A 95% yield of electrophoretically pure anti-GM-CSF was obtained in a single step. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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