Accumulation of deacetoxycephalosporin C by a deacetylcephalosporin C negative mutant of Cephalosporium acremonium
| Autor: | Toshihiko Kanzaki, Yukio Fujisawa, Kazuaki Kitano |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Strain (chemistry)
Stereochemistry medicine.drug_class Chemistry Mutant Cephalosporin Deacetoxycephalosporin C General Biochemistry Genetics and Molecular Biology De novo synthesis chemistry.chemical_compound Biosynthesis Cephalosporium acremonium medicine Fermentation General Agricultural and Biological Sciences |
| Zdroj: | Agricultural and Biological Chemistry. 39:2049-2055 |
| ISSN: | 1881-1280 0002-1369 |
| DOI: | 10.1271/bbb1961.39.2049 |
| Popis: | Deacetylcephalosporin C negative mutants, lacking a certain step in the pathway of deacetylcephalosporin C biosynthesis, were obtained from the deacetylcephalosporin C producing mutant No. 40 of Cephalosporium acremonium by treatment with N-methyl-N′-nitro-N-nitrosoguanidine. Among these mutants, the strain No. 40-20 was found to mainly accumulate a cephalosporin compound other than deacetylcephalosporin C and cephalosporin C. The cephalosporin was isolated as crystals from the culture broth of the mutant No. 40-20, and identified as deacetoxycephalosporin C, possessing a D-a-aminoadipyl side chain at C-7, by physical, chemical and biological methods. The profile of deacetoxycephalosporin C fermentation and the examination of the biochemical reduction of deacetylcephalosporin C led us to the conclusion that deacetoxycephalosporin C would be produced through de novo synthesis by this mutant. |
| Databáze: | OpenAIRE |
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