| Autor: |
Trishna Gautom, Dharmendra Dheeman, Colin Levy, Thomas Butterfield, Lewis Caiger, Karl Fisher, Linus Johannissen, Neil Dixon |
| Rok vydání: |
2021 |
| DOI: |
10.21203/rs.3.rs-358367/v1 |
| Popis: |
Biological degradation of Polyethylene terephthalate (PET) plastic and assimilation of the corresponding monomers ethylene glycol and terephthalate (TPA) into central metabolism offers an attractive route for bio-based molecular recycling and bioremediation applications. A key step is the cellular uptake of the non-permeable TPA into bacterial cells which has been shown to be dependent upon the presence of the key tphC gene. However, little is known from a biochemical and structural perspective about the encoded solute binding protein, TphC. Here, we report the biochemical and structural characterisation of TphC in both open and TPA-bound closed conformations. This analysis demonstrates the narrow ligand specificity of TphC towards aromatic para-substituted dicarboxylates, such as TPA and closely related analogues. Further phylogenetic and genomic context analysis of the tph genes reveals homologous operons as a genetic resource for future biotechnological and metabolic engineering efforts towards circular plastic bio-economy solutions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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