UnyLinker: An Efficient and Scaleable Synthesis of Oligonucleotides Utilizing a Universal Linker Molecule: A Novel Approach To Enhance the Purity of Drugs

Autor:	Phil Olsen, R. Krishna Kumar, Lucio Mendez, Anthony N. Scozzari, Max N. Moore, Zhiwei Wang, Recaldo L. Carty, Vasulinga T. Ravikumar, Isaiah Cedillo, Ratnasamy Somanathan, Xue-Feng Zhu, Mark Andrade, Gerardo Aguirre, Dennis Gorman, Sylvain Berneès
Rok vydání:	2008
Předmět:	Chemistry Oligonucleotide Stereochemistry Organic Chemistry Nucleic acid Molecule Physical and Theoretical Chemistry Oligonucleotide synthesis Branching (polymer chemistry) Linker Vicinal Conjugate
Zdroj:	Organic Process Research & Development. 12:399-410
ISSN:	1520-586X 1083-6160
DOI:	10.1021/op8000178
Popis:	A novel universal linker (UnyLinker) molecule which has a conformationally rigid and chemically stable bridge head ring oxygen atom carrying a conventional 4,4′-dimethoxytrityl (DMT) and succinyl groups locked in a syn orientation has been developed to carry out oligonucleotide synthesis efficiently and smoothly. The geometry of the vicinal syn oxygen functionalized group allows fast and clean cleavage under standard aqueous ammonia deprotection conditions to afford high-quality oligonucleotides. No base modification is observed, based on the ion-pair HPLC−UV−MS (IP-HPLC−UV−MS) method with detection limit of
Databáze:	OpenAIRE
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