Screening and evaluation of antioxidants from lees by micro-injector systems combined with a fluorescent probe, N-borylbenzyloxycarbonyl-3,7-dihydroxyphenoxazine, in living Drosophila

Autor: Hou Xiaotao, Wenjuan Liu, Gang Bai, Hongjun Wei, Guangcui Chu, Dandan Cheng, Jiagang Deng, Yuanyuan Hou, Xuefeng Wang
Rok vydání: 2018
Předmět:
Zdroj: Chinese Chemical Letters. 29:1521-1527
ISSN: 1001-8417
DOI: 10.1016/j.cclet.2018.05.020
Popis: Over the past few decades, the determination of antioxidant activity by chemical probe has been widely reported, but in vivo evaluation via model organisms of Drosophila melanogaster and rapid discovery system has not been studied adequately. In this study, we determined the antioxidant activity of lees and demonstrated the ability of compounds in lees to scavenge H2O2 in vitro and in vivo by different chemical probes. Lees increased the ability of Drosophila against oxidative stress and antioxidant enzyme activity in vivo. Five ingredients of organic acids and flavones in lees extract that rapidly scavenged H2O2 were revealed by a post-column-derived HPLC-UV-FLD system based on 4-hydroxyphenylacetic acid (PHPAA) chemiluminescence. Additionally, another fluorescent probe, N-borylbenzyloxycarbonyl-3,7-dihydroxyphenoxazine (NBCD), was selected to evaluate the reactive oxygen species (ROS) scavenging capacity of lees in living Drosophila melanogaster using a microfluidic injection test coupled with microscopic imaging analysis, and similar effects were observed in flies when they were treated with tartaric acid and caffeic acid. The results demonstrated that the novel integrated system was suitable for screening and evaluating antioxidant ingredients from natural products.
Databáze: OpenAIRE
Externí odkaz: