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Purpose To investigate whether there is any difference in the safety, feasibility and intraoperative lymph node clearance of the Da Vinci robot-assisted surgery in endometrial cancer lymph node dissection compared to conventional laparoscopic surgery. Methods A retrospective analysis was made on the clinical data of 83 patients with endometrial cancer enrolled in the Affiliated Hospital of Jining Medical University from January 2018 to March 2022. 41 patients received Da Vinci robot-assisted surgery (RAS group) and 42 received conventional laparoscopic surgery (CLS group). To collect and compare the general condition, operation time, intraoperative bleeding, presence of intraoperative injury, postoperative exhaustion time, application of antibiotics, duration of pelvic drainage tube retention, duration of indwelling catheter, whether postoperative pain medication was applied, whether postoperative abdominal distension was present, number of intraoperative lymph nodes cleared and positive, and LNR between the two groups. Results There was a statistically significant difference (P 0.05) in terms of intraoperative bleeding, postoperative time to exhaustion, time to apply antibiotics, time to leave a pelvic drain in place, time to leave a catheter in place, length of hospital stay, whether pain medication was applied postoperatively, and whether there was postoperative abdominal distension. When comparing the number of lymph nodes cleared intraoperatively, the number of positives and LNR between the two groups, the differences were statistically significant (P 0.05), while the differences were statistically significant (P |