Imaging in fluorescence direct-view microscopy
| Autor: | S. J. Hewlett, D. T. Fewer, Eithne M. McCabe |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science Microscope Optical sectioning business.industry Laser Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials law.invention Optics law Light sheet fluorescence microscopy Optical transfer function Microscopy Fluorescence microscope Electrical and Electronic Engineering Physical and Theoretical Chemistry business |
| Zdroj: | Optics Communications. 152:393-402 |
| ISSN: | 0030-4018 |
| DOI: | 10.1016/s0030-4018(98)00174-6 |
| Popis: | We derive a general theory of imaging for the fluorescence direct-view microscope (DVM) in terms of the system's incoherent optical transfer function. Particular attention is given to both the form of the in-focus transfer function and the optical sectioning capability of the instrument in the presence of (i) a spatially incoherent source and (ii) a spatially coherent (laser) source. Unlike its brightfield counterpart, the imaging properties of the fluorescence DVM are shown to be relatively insensitive to the source coherence, provided the pinhole spacing-to-radius ratio is kept sufficiently large. This suggests that the use of a laser source in fluorescence direct-view microscopy could prove to be extremely beneficial as regards increased light throughput and cost-effectiveness. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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