| Autor: |
Eric A. Kitas, E. J. Schlaeger, K. Christensen, Ulrich Deuschle, Arnold Trzeciak, Andreas Supersaxo, Jean-Yves Legendre |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
New Developments and New Applications in Animal Cell Technology ISBN: 0792350162 |
| DOI: |
10.1007/0-306-46860-3_18 |
| Popis: |
The aim of the presented work was to develop a cost-effective and easily scaleable transient transfection system with mammalian cells grown in serum-free suspension culture. For this purpose the cationic polyethylenimine (PEI) and the novel hybrid molecule dioleoyl-melittin (DOM) were used. Both substances are highly efficient transfection reagents for mammalian cells and have been described recently. The transfection complexes were made directly within the cell culture by consecutively adding plasmid and PEI (direct method). Alternatively, the DNA-PEI transfection complexes were performed in fresh medium (1/10 culture volume) and then added to the cells (indirect method). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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